217 DIFFERENTIATION OF PLURIPOTENT HUMAN STEM CELLS TO EPICARDIUM AND EPICARDIUM-DERIVED CORONARY SMOOTH MUSCLE CELLS
نویسندگان
چکیده
منابع مشابه
Notch signaling regulates smooth muscle differentiation of epicardium-derived cells.
RATIONALE The embryonic epicardium plays a crucial role in the formation of the coronary vasculature and in myocardial development, yet the exact contribution of epicardium-derived cells (EPDCs) to the vascular and connective tissue of the heart, and the factors that regulate epicardial differentiation, are insufficiently understood. OBJECTIVE To define the role of Notch signaling in murine e...
متن کاملRobust derivation of epicardium and its differentiated smooth muscle cell progeny from human pluripotent stem cells
The epicardium has emerged as a multipotent cardiovascular progenitor source with therapeutic potential for coronary smooth muscle cell, cardiac fibroblast (CF) and cardiomyocyte regeneration, owing to its fundamental role in heart development and its potential ability to initiate myocardial repair in injured adult tissues. Here, we describe a chemically defined method for generating epicardium...
متن کاملCellular Biology Notch Signaling Regulates Smooth Muscle Differentiation of Epicardium-Derived Cells
Rationale: The embryonic epicardium plays a crucial role in the formation of the coronary vasculature and in myocardial development, yet the exact contribution of epicardium-derived cells (EPDCs) to the vascular and connective tissue of the heart, and the factors that regulate epicardial differentiation, are insufficiently understood. T he epicardium, the outermost layer of the heart, develops ...
متن کاملReprogramming by cytosolic extract of human embryonic stem cells improves dopaminergic differentiation potential of human adipose tissue-derived stem cells
The extract of pluripotent stem cells induces dedifferentiation of somatic cells with restricted plasticity. In this study, we used the extract of human embryonic stem cells (hESC) to dedifferentiate adipose tissue-derived stem cells (ADSCs) and examined the impact of this reprogramming event on dopaminergic differentiation of the cells. For this purpose, cytoplasmic extract of ESCs was prepare...
متن کاملIsolation and Characterization of Human Induced Pluripotent Stem Cells-Derived Mesenchymal Progenitors
Purpose: Isolating human induced pluripotent stem cells (hiPS)-derived mesenchymal progenitors as a new source of mesenchymal cells which can differentiate into different lineages like adipose and bone. Materials and Methods: After 7 days of hiPS1 culture on matrigle coated dishes, spindle like cells around colonies were removed by cell scraper. These cells that had mesenchymal like morphology ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Heart
سال: 2013
ISSN: 1355-6037,1468-201X
DOI: 10.1136/heartjnl-2013-304019.217